Аннотация:
Aim: to study effect of combined administration of diazepam and pterostilbene, analog of resveratrol, on development of seizures, as well as on intensity of lipid peroxidation (LPO) processes in brain of rats exposed to maximal electroshock (MES).
Methods. The experiments were carried out on male Sprague Dawley rats. The intensity of LPO processes was recorded using spectrophotometric method. Anticonvulsant effect of studied substances was estimated by the ability to prevent or shorten duration of tonic hind limb extension.
Results. The MES procedure induced development of pronounced convulsive seizures of clonic-tonic type in 100% of animals. Diazepam at high dose near completely stopped phase of tonic hind limb extension of seizures caused by MES. However, expressed drowsiness, lethargy, and decreased locomotor activity were noted in the animals of this group. Diazepam at low dose, as well as natural antioxidant pterostilbene, did not have a significant effect on duration of tonic extension in rats. Simultaneous administration of low dose diazepam and pterostilbene resulted in almost complete inhibition of the tonic extension phase evoked by MES procedure. Diazepam at high dose partially reduced intensification of LPO processes caused by MES. Pterostilbene led to even more pronounced decrease in the level of LPO products in the cerebral cortex of rats, compared to that of control animals. The most pronounced decrease in level of LPO products induced by MES was observed with simultaneous administration of diazepam in low dose and pterostilbene.
Conclusion. Summarizing our results and data of literature it could be further consider possibility in including of combination of low doses of diazepam with pterostilbene in complex therapy.
Ключевые слова:
anticonvulsant effect, maximal electroshock; lipid peroxidation; diazepam, pterostilbene