Аннотация:
The aim of this work was to determine how chronic alcoholization and alcohol withdrawal affect the stabilization of the genome and the content of orexin in brain structures in rats. To determine the genotoxic damage to peripheral blood cells, a micronucleus test was used. It has been shown that chronic alcoholization is accompanied by an increase in the number of micronuclei in peripheral blood mononuclear cells, an indicator of genome destabilization. Chronic alcoholism and 2-day withdrawal of ethanol cause an increase in the number of micronuclei in peripheral blood mononuclear cells, realizing indicators of genome destabilization. At the same time, 7 days after the cancellation, the indicators no longer differ from those observed in the control group. Previously, we have shown that the orexin system is involved in the stabilization of the cellular genome during chronic alcoholization in rats. In this regard, in this work, using enzyme immunoassay, the content of orexin A was studied in chronic alcoholization and alcohol withdrawal. In the control groups of rats, the concentration of orexin A in the hypothalamus did not differ from its concentration in the amygdala and hippocampus. A similar picture was observed during chronic alcoholization. In the control groups of rats, the concentration of orexin A in the hypothalamus did not differ from its concentration in the amygdala and hippocampus. A similar picture was observed during chronic alcoholization. In the 2-day and 7-day withdrawal groups, the concentration of orexin A in the amygdala was significantly increased compared to the control group of rats and rats with chronic alcoholism. The concentration of orexin A in the amygdala was also significantly increased compared to that in the hippocampus and hypothalamus during 2-day and 7-day alcohol withdrawal. It is concluded that the amygdala complex is an intracerebral target for the study and search for drugs that affect the brain orexin system and genome stabilization in alcoholism and its withdrawal.
Ключевые слова:
genome destabilization, orexin, brain structures, chronic alcoholization, experimental study.