Аннотация:
Introduction. One of the severe conditions developing in the structure of alcohol withdrawal syndrome is seizures. Due to the fact that with chronic alcohol intoxication, the neurobiological regulation of circadian rhythms is disrupted, contributing to the disruption of the activity of the polygenic network, the risk of seizures increases. We believe that genes regulating circadian rhythms and their polymorphic variants caused by mutations increase the risk of seizures.
Aim: to determine the association of single-nucleotide variants of circadian genes and seizures after alcohol withdrawal.
Methods. The study included 399 patients, of whom 83 were women (21%) and 316 were men (79%). The average age of the subjects was 41.52±8.42 years. All patients were divided into two groups: the first group included patients who had suffered a convulsive attack in a hospital setting (n=108); the comparison group was patients without convulsive seizures in a hospital setting and in anamnesis (n=291). Genotyping of the HTR2A (rs6313), MTNR1A (rs34532313), MTNR1B (rs10830963), CLOCK (rs1801260), PER2 (rs934945), CRY1 (rs1800497) genes was performed using real-time polymerase chain reaction (RT-PCR). Statistical processing was carried out using the Pearson χ² test, as well as assessing the odds ratio for each genotype. The differences were considered statistically significant at a significance level of p<0.05.
Results. As a result of the study, it was revealed that there is an association of the CLOCK gene and seizures after alcohol withdrawal in patients with alcohol dependence syndrome. There were no associations with other genes regulating circadian rhythms.
Conclusions. The presence of the CLOCK gene T allele in the genotype of patients is associated with seizures after alcohol withdrawal, and is a risk of seizures with complications of alcohol withdrawal syndrome.
Ключевые слова:
alcoholism, CLOCK, seizures, alcohol, circadian rhythms, alcohol withdrawal syndrome.