Аннотация:
Prolonged alcohol exposure activates TLR4-signaling pathways in the brain, which is responsible for the development of neuroinflammation. There is interest in pharmacologic correction of these mechanisms. The antibiotic rifampicin (Rif) has established itself as a potential neuroprotectant in studies on its use to correct various pathologic conditions of the nervous system associated with the development of neuroinflammatory events. We performed a study on human neuroblastoma cell culture SH-SY5Y. Prolonged incubation of cells in ethanol (24 h, 100 mM) of SH-SY5Y induces activation of the innate immune system genes Tlr4 and Il1β. Pre-addition of rifampicin (25-100 mM) prior to incubation of cells in ethanol solution inhibited Tlr4 and Il1β gene expression, whereas addition of rifampicin after incubation of cells in ethanol dose-dependently reduced the increased expression of Tlr4 and Il1β genes, with the most significant effect at a concentration of 100 mM. In addition, the use of rifampicin increased cell survival in culture. Thus, the results of our experiment showed that Rif is able to eliminate the increased expression of inflammation genes caused by prolonged alcohol exposure and to increase the survival rate of long-term incubated cells in ethanol solution.
Ключевые слова:
ethanol, neuroinflammation, TLR4, SH-SY5Y, rifampicin.